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Rabbit anti-SMG1 Antibody, Affinity Purified - A300-394A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-SMG1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-394A
Name:
Rabbit anti-SMG1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to SMG1 immobilized on solid support. The epitope recognized by A300-394A maps to a region between residues 2925 and 2975 of human PI-3-Kinase-related kinase SMG1 using the numbering given in entry NP_055907.3 (GeneID 23049).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
61E3.4, ATX, hSMG-1, lambda/iota protein kinase C-interacting protein, lambda-interacting protein, LIP, PI-3-kinase-related kinase SMG-1, serine/threonine-protein kinase SMG1, SMG-1, smg-1 homolog, phosphatidylinositol 3-kinase-related kinase, SMG1 phosph
Species Reactivity:
Human
Immunogen:
Between 2925 and 2975
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human SMG1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody A300-394A (lot A300-394A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

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Detection of human SMG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMG1 antibody A300-394A (lot A300-394A-3) used for IP at 6 µg per reaction. SMG1 was also immunoprecipitated by a previous lot of this antibody (lot A300-394A-2). For blotting immunoprecipitated SMG1, A300-394A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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