Antibody was affinity purified using an epitope specific to SMG1 immobilized on solid support. The epitope recognized by A300-393A maps to a region between residues 1625 and 1675 of human PI-3-Kinase-related kinase SMG1 using the numbering given in entry NP_055907.3 (GeneID 23049). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
61E3.4, ATX, hSMG-1, lambda/iota protein kinase C-interacting protein, lambda-interacting protein, LIP, PI-3-kinase-related kinase SMG-1, serine/threonine-protein kinase SMG1, SMG-1, smg-1 homolog, phosphatidylinositol 3-kinase-related kinase, SMG1 phosph
between 1625 and 1675
2 - 8°C
Detection of human SMG1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMG1 antibody A300-393A (lot A300-393A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.