Antibody was affinity purified using an epitope specific to DHX15 immobilized on solid support. The epitope recognized by A300-390A maps to a region between residue 750 and the C-terminus (residue 795) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATP-dependent RNA helicase #46, DBP1, DDX15, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15, DEAD/H box-15, DEAH (Asp-Glu-Ala-His) box helicase 15, DEAH (Asp-Glu-Ala-His) box polypeptide 15, DEAH box protein 15, HRH2, pre-mRNA-splicing factor ATP-depende
between 750 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human DHX15 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody A300-390A (lot A300-390A-1) used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human DHX15 by western blot and immunoprecipitation. Samples: Whole cell lysate (CL; 60 µg for WB, 1 mg for IP) from HEK293T cells. Antibodies: Affinity purified rabbit anti-DHX15 antibody BL1628 (Cat. No. A300-390A) used at 0.1 µg/ml for WB and at 1 µg/mg lysate for IP. immunoprecipitatesd DHX15 was blotted using affinity purified rabbit anti-DHX15 antibody BL1627 (Cat. No. A300-389A) at 0.1 µg/ml. BL1627 was also used unsuccessfully for IP (1 µg/mg lysate). Detection: Chemiluminescence with an exposure time of 15 seconds.