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Rabbit anti-EGFR Antibody, Affinity Purified - A300-388A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-EGFR Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-388A
Name:
Rabbit anti-EGFR Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to EGFR immobilized on solid support. The epitope recognized by A300-388A maps to a region between residue 1150 and the C-terminus (residue 1210) of human Epidermal Growth Factor Receptor using the numbering given in Swiss-Prot entry P00533 (GeneID 1956).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor tyrosine kinase domain, ERBB, ERBB1, erb-b2 recep
Species Reactivity:
Human, Mouse
Immunogen:
Between 1150 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human EGFR by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody A300-388A (lot A300-388A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human and mouse EGFR by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody A300-388A (lot A300-388A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human EGFR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EGFR antibody A300-388A (lot A300-388A-2) used for IP at 6 µg per reaction. EGFR was also immunoprecipitated by rabbit anti-EGFR antibody A300-387A. For blotting immunoprecipitated EGFR, A300-388A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Affinity purified rabbit anti-EGFR (Cat. No. A300-388A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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