Antibody was affinity purified using an epitope specific to EGFR immobilized on solid support. The epitope recognized by A300-387A maps to a region between residues 1100 and 1150 of human Epidermal Growth Factor Receptor using the numbering given in Swiss-Prot entry P00533 (GeneID 1956). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor tyrosine kinase domain, ERBB, ERBB1, erb-b2 recep
between 1100 and 1150
2 - 8°C
Detection of human EGFR by western blot. Samples: Whole cell lysate (50 µg) from HeLa and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EGFR antibody A300-387A (lot A300-387A-1) used for WB at 0.02 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.