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Rabbit anti-BubR1 Antibody, Affinity Purified

Catalogue number:
A300-386A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to BubR1 immobilized on solid support. The epitope recognized by A300-386A maps to a region between residues 350 and 400 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in TrEMBL entry Q8WV50 (GeneID 701). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

Bub1A, BUB1B, mitotic checkpoint serine/threonine kinase, BUB1beta, BUBR1, budding uninhibited by benzimidazoles 1 homolog beta, hBUBR1, MAD3/BUB1-related protein kinase, MAD3L, mitotic checkpoint kinase MAD3L, mitotic checkpoint serine/threonine-protein

Species Reactivity:

Human, Mouse, Rat

Immunogen:

between 350 and 400

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human BubR1 by western blot

Detection of human BubR1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody A300-386A (lot A300-386A-2) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Detection of mouse and rat BubR1 by west

Detection of mouse and rat BubR1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody A300-386A (lot A300-386A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.