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Rabbit anti-BubR1 Antibody, Affinity Purified - A300-386A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-BubR1 Antibody, Affinity Purified
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Size: 100 µg
£309.00
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Catalogue number:
A300-386A
Name:
Rabbit anti-BubR1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to BubR1 immobilized on solid support. The epitope recognized by A300-386A maps to a region between residues 350 and 400 of human budding uninhibited by benzimidazoles 1 homolog beta using the numbering given in TrEMBL entry Q8WV50 (GeneID 701).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
Bub1A, BUB1B, mitotic checkpoint serine/threonine kinase, BUB1beta, BUBR1, budding uninhibited by benzimidazoles 1 homolog beta, hBUBR1, MAD3/BUB1-related protein kinase, MAD3L, mitotic checkpoint kinase MAD3L, mitotic checkpoint serine/threonine-protein
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Between 350 and 400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human BubR1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody A300-386A (lot A300-386A-2) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

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Detection of mouse and rat BubR1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-BubR1 antibody A300-386A (lot A300-386A-2) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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