Antibody was affinity purified using an epitope specific to Bcl11b immobilized on solid support. The epitope recognized by A300-384A maps to a region between residues 500 and 550 of human B-cell CLL/lymphoma 11B using the numbering given in entry NP_612808.1 (GeneID 64919). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATL1, ATL1-alpha, ATL1-beta, ATL1-delta, ATL1-gamma, B-cell CLL/lymphoma 11B, B-cell CLL/lymphoma 11B (zinc finger protein), B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein, B-cell lymphoma/leukaemia 11B, B-cell lymphoma/leuke
between 500 and 550
2 - 8°C
Detection of human Bcl11b by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody A300-384A (lot A300-384A-1) used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Bcl11b by western blot and immunoprecipitation. Samples: Nuclear extract (5 and 50 µg for WB, 1 mg for IP) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl11b antibody BL1800 (Cat. No. A300-384A) used at 0.1 µg/ml (A) and 0.5 µg/ml (B) for WB and at 1 and 10 µg/mg lysate for IP (B). Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies BL1798 (Cat. No. A300-383A) and BL1801 (Cat. No. A300-385A) at 1 and 10 µg/mg lysate. Normal rabbit IgG was used as a control for immunoprecipitation reactions. Detection: Chemiluminescence with exposure times of 1 minute (A), 1 second (B) or 30 seconds (B).