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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-MLL1 Antibody, Affinity Purified
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Rabbit anti-MLL1 Antibody, Affinity Purified

Catalogue number:
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A300-374A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
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  • Rest of World
£436.00
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Estimated delivery Wed 1 May
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Applications:

WB, IP, ChIP, ChIP-chip

Antibody Host:

Rabbit

Target:

MLL1

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 2725 and 2775

Alternative Names:

MLL1; WDSTS; TRX1; trithorax-like protein; Myeloid/lymphoid or mixed-lineage leukemia protein 1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); Myeloid/lymphoid or mixed-lineage leukemia; MLL1A; lysine (K)-specific methyltrans

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Product Description:

Rabbit anti-MLL1 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human MLL1 by western blot.
Detection of human MLL1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLL1 antibody A300-374A (lot A300-374A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MLL1 by western blot
Detection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLL1 antibody A300-374A (lot A300-374A-5) used for IP at 3 µg per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody BL1412. For blotting immunoprecipitated MLL1, A300-374A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
ChIP-chip scatter plot of anti-MLL1 (A30
ChIP-chip scatter plot of anti-MLL1 (A300-374A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-374A was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitatesd DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-MLL1 ChIP, normal rabbit IgG showed little enrichment.
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