Rabbit anti-PTIP Antibody, Affinity Purified - A300-370A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to PTIP immobilized on solid support. The epitope recognized by A300-370A maps to a region between residues 1 and 50 of human PAX transcription activation domain interacting protein 1 using the numbering given in Jowsey, Doherty and Rouse, 2004, J. Biol. Chem. 279(53):55562-55569 (GeneID 22976).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human PTIP by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PTIP antibody A300-370A (lot A300-370A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PTIP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PTIP antibody A300-370A (lot A300-370A-2) used for IP at 3 µg per reaction. PTIP was also immunoprecipitated by rabbit anti-PTIP antibody BL1788. For blotting immunoprecipitated PTIP, A300-370A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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