Rabbit anti-CBP Antibody, Affinity Purified - A300-362A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to CBP immobilized on solid support. The epitope recognized by A300-362A maps to a region between residues 975 and 1025 of human CREB Binding Protein using the numbering given in entry NP_004371.1 (GeneID 1387). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human CBP by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody A300-362A (lot A300-362A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human CBP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody A300-362A (lot A300-362A-1) used for IP at 6 µg/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody A300-363A. For blotting immunoprecipitated CBP, A300-362A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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