Antibody was affinity purified using an epitope specific to CA150 immobilized on solid support. The epitope recognized by A300-360A maps to a region between residues 1 and 50 of human Transcription Factor CA150 (Transcription Elongation Regulator 1) using the numbering given in entry NP_006697.2 (GeneID 10915). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CA150, co-activator of 150 kDa, TAF2S, TATA box binding protein (TBP)-associated factor, RNA polymerase II, S, 150kD, TATA box-binding protein-associated factor 2S, transcription elongation regulator 1, transcription factor CA150, Urn1
between 1 and 50
2 - 8°C
Detection of human CA150 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CA150 antibody A300-360A (lot A300-360A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human and mouse CA150 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-CA150 (Cat. No. A300-360A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB