Antibody was affinity purified using an epitope specific to p300 immobilized on solid support. The epitope recognized by A300-359A maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E1A-associated protein p300, E1A-binding protein, 300kD, histone acetyltransferase p300, histone butyryltransferase p300, histone crotonyltransferase p300, KAT3B, p300, p300 HAT, protein propionyltransferase p300, RSTS2
between 1000 and 1050
2 - 8°C
Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody A300-359A (lot A300-359A-2) used for IP at 6 µg/mg lysate. p300 was also immunoprecipitated by a previous lot (lot A300-359A-1) of this antibody. For blotting immunoprecipitated p300, A300-359A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.