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Rabbit anti-p300 Antibody, Affinity Purified

Catalogue number:
A300-359A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to p300 immobilized on solid support. The epitope recognized by A300-359A maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Product Type:

Antibodies

Alternative Names:

E1A-associated protein p300, E1A-binding protein, 300kD, histone acetyltransferase p300, histone butyryltransferase p300, histone crotonyltransferase p300, KAT3B, p300, p300 HAT, protein propionyltransferase p300, RSTS2

Species Reactivity:

Human

Immunogen:

between 1000 and 1050

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human p300 by western blot.

Detection of human p300 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody A300-359A (lot A300-359A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human p300 by western blot

Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody A300-359A (lot A300-359A-2) used for IP at 6 µg/mg lysate. p300 was also immunoprecipitated by a previous lot (lot A300-359A-1) of this antibody. For blotting immunoprecipitated p300, A300-359A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human p300 by immunohistoch

Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 (Cat. No. A300-359A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB