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Rabbit anti-p300 Antibody, Affinity Purified - A300-359A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-p300 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-359A
Name:
Rabbit anti-p300 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to p300 immobilized on solid support. The epitope recognized by A300-359A maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
E1A-associated protein p300, E1A-binding protein, 300kD, histone acetyltransferase p300, histone butyryltransferase p300, histone crotonyltransferase p300, KAT3B, p300, p300 HAT, protein propionyltransferase p300, RSTS2
Species Reactivity:
Human
Immunogen:
Between 1000 and 1050
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human p300 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-p300 antibody A300-359A (lot A300-359A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-p300 antibody A300-359A (lot A300-359A-2) used for IP at 6 µg/mg lysate. p300 was also immunoprecipitated by a previous lot (lot A300-359A-1) of this antibody. For blotting immunoprecipitated p300, A300-359A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

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Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- p300 (Cat. No. A300-359A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

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