Antibody was affinity purified using an epitope specific to p300 immobilized on solid support. The epitope recognized by A300-358A maps to a region between residues 950 and 1000 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E1A-associated protein p300, E1A-binding protein, 300kD, histone acetyltransferase p300, histone butyryltransferase p300, histone crotonyltransferase p300, KAT3B, p300, p300 HAT, protein propionyltransferase p300, RSTS2
Between 950 and 1000
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human p300 by western blot. Samples: Whole cell lysate from HeLa (15, 5 µg), HEK293T (15, 5 µg), and Jurkat (50 µg)cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p300 antibody A300-358A (lot A300-358A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p300 antibody A300-358A (lot A300-358A-4) used for IP at 3 µg per reaction. p300 was also immunoprecipitated by rabbit anti-p300 antibody A300-359A. For blotting immunoprecipitated p300, A300-358A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.