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Rabbit anti-p300 Antibody, Affinity Purified - A300-358A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-p300 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-358A
Name:
Rabbit anti-p300 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to p300 immobilized on solid support. The epitope recognized by A300-358A maps to a region between residues 950 and 1000 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC, ChIP-Seq

Product Type:
Antibodies
Alternative Names:
E1A-associated protein p300, E1A-binding protein, 300kD, histone acetyltransferase p300, histone butyryltransferase p300, histone crotonyltransferase p300, KAT3B, p300, p300 HAT, protein propionyltransferase p300, RSTS2
Species Reactivity:
Human
Immunogen:
Between 950 and 1000
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human p300 by western blot. Samples: Whole cell lysate from HeLa (15, 5 µg), HEK293T (15, 5 µg), and Jurkat (50 µg)cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p300 antibody A300-358A (lot A300-358A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

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Detection of human p300 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p300 antibody A300-358A (lot A300-358A-4) used for IP at 3 µg per reaction. p300 was also immunoprecipitated by rabbit anti-p300 antibody A300-359A. For blotting immunoprecipitated p300, A300-358A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 (Cat. No. A300-358A Lot 4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

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