Antibody was affinity purified using an epitope specific to PAK4 immobilized on solid support. The epitope recognized by A300-356A maps to a region between residue 25 and 75 of human p21-Activated Kinase 4 using the numbering given in entry NP_005875.1 (GeneID 10298). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
p21 protein (Cdc42/Rac)-activated kinase 4, p21(CDKN1A)-activated kinase 4, p21-activated kinase 4, PAK-4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, serine/threonine-protein kinase PAK 4
between 25 and 75
2 - 8°C
Detection of human and mouse PAK4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PAK4 antibody A300-356A (lot A300-356A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PAK4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAK4 antibody A300-356A (lot A300-356A-2) used for IP at 6 µg per reaction. PAK4 was also immunoprecipitated by rabbit anti-PAK4 antibody BL512. For blotting immunoprecipitated PAK4, A300-356A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.