Antibody was affinity purified using an epitope specific to Galectin-7 immobilized on solid support. The epitope recognized by A300-354A maps to a region between residue 100 and the C-terminus (residue 135) of human Galectin-7 using the numbering given in entry NP_002298.1 (GeneID 3963). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
GAL7, Gal-7, galectin-7, HKL-14, lectin, galactoside-binding, soluble, 7, LGALS7A, p53-induced gene 1 protein, PI7
between 100 and C-term
2 - 8°C
Detection of mouse Galectin 7 by western blot and immunoprecipitation. Samples: A. Modified RIPA extract (10 µg) from the epidermis of FVB wild-type (wt) or COX2 transgenic (TG) mice. B. Modified RIPA extract (20 µg) from the epidermis of FVB wild-type mice. Antibody: Affinity purified rabbit anti-galectin 7 (Cat. No. A300-354A) used at 0.005 (A) or 0.0025 (B) µg/ml for WB and as indicated for IP. Detection: Chemiluminescence with 1 second exposure time.