Antibody was affinity purified using an epitope specific to E6AP immobilized on solid support. The epitope recognized by A300-351A maps to a region between residue 75 and 125 of human Ubiquitin Protein Ligase E3A using the numbering given in entry NP_000453.2 (GeneID 7337). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ANCR, AS, CTCL tumor antigen se37-2, E6-AP, E6AP ubiquitin-protein ligase, EPVE6AP, HECT-type ubiquitin transferase E3A, HPVE6A, human papilloma virus E6-associated protein, human papillomavirus E6-associated protein, oncogenic protein-associated protein
between 75 and 125
2 - 8°C
Detection of human and mouse E6AP by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-E6AP antibody A300-351A (lot A300-351A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human E6AP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-E6AP antibody A300-351A (lot A300-351A-2) used for IP at 6 µg/mg lysate. E6AP was also immunoprecipitated by a previous lot of this antibody (lot A300-351A-1), and rabbit anti-E6AP antibody A300-352A. For blotting immunoprecipitated E6AP, A300-351A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.