Antibody was affinity purified using an epitope specific to NCOA2/SRC2 immobilized on solid support. The epitope recognized by A300-346A maps to a region between residue 1400 the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bHLHe75, class E basic helix-loop-helix protein 75, glucocorticoid receptor-interacting protein-1, GRIP1, hTIF2, KAT13C, NCoA-2, nuclear receptor coactivator 2, p160 steroid receptor coactivator 2, SRC2, TIF2, transcriptional intermediary factor 2
between 1400 and C-term
2 - 8°C
Detection of human NCOA2/SRC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa(15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody A300-346A (lot A300-346A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NCOA2/SRC2 was also immunoprecipitated by a previous lot (A300-346A-1) and by rabbit anti-NCOA2/SRC2 antibody A300-345A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of mouse NCOA2/ SRC2 by western blot. Samples: Whole cell lysate (50 µg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2/SRC2 antibody A300-346A (lot A300-346A-1) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human NCOA2/ SRC2 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-NCOA2/ SRC2 (Cat. No. A300-346A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB