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Rabbit anti-SRC1 Antibody, Affinity Purified

Catalogue number:
A300-343A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to SRC1 immobilized on solid support. The epitope recognized by A300-343A maps to a region between residues 600 and 650 of human Nuclear Receptor Coactivator 1 using the numbering given in entry NP_003734.3 (GeneID 8648). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

bHLHe42, bHLHe74, class E basic helix-loop-helix protein 74, F-SRC-1, Hin-2 protein, KAT13A, NCoA-1, nuclear receptor coactivator 1, Protein Hin-2, renal carcinoma antigen NY-REN-52, RIP160, SRC1, SRC-1, Steroid receptor coactivator 1, steroid receptor co

Species Reactivity:

Human, Mouse

Immunogen:

between 600 and 650

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse SRC1 by wes

Detection of human and mouse SRC1 by western blot. Samples: Whole cell lysate from HEK293T (50, 15, 5 µg), and (50 µg) from HeLa, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody A300-343A (lot A300-343A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human SRC1 by western blot

Detection of human SRC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRC1 antibody A300-343A (lot A300-343A-2) used for IP at 6 µg per reaction. SRC1 was also immunoprecipitated by a previous lot of this antibody A300-343A-1. For blotting immunoprecipitated SRC1, A300-343A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human SRC1 by immunohistoch

Detection of human SRC1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SRC1 (Cat. No. A300-343A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB