Antibody was affinity purified using an epitope specific to SRC1 immobilized on solid support. The epitope recognized by A300-343A maps to a region between residues 600 and 650 of human Nuclear Receptor Coactivator 1 using the numbering given in entry NP_003734.3 (GeneID 8648). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bHLHe42, bHLHe74, class E basic helix-loop-helix protein 74, F-SRC-1, Hin-2 protein, KAT13A, NCoA-1, nuclear receptor coactivator 1, Protein Hin-2, renal carcinoma antigen NY-REN-52, RIP160, SRC1, SRC-1, Steroid receptor coactivator 1, steroid receptor co
between 600 and 650
2 - 8°C
Detection of human and mouse SRC1 by western blot. Samples: Whole cell lysate from HEK293T (50, 15, 5 µg), and (50 µg) from HeLa, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC1 antibody A300-343A (lot A300-343A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human SRC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SRC1 antibody A300-343A (lot A300-343A-2) used for IP at 6 µg per reaction. SRC1 was also immunoprecipitated by a previous lot of this antibody A300-343A-1. For blotting immunoprecipitated SRC1, A300-343A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.