Antibody was affinity purified using an epitope specific to MAD1 immobilized on solid support. The epitope recognized by A300-339A maps to a region between residues 1 and 50 of human Mitotic arrest deficient-like 1 using the numbering given in Swiss-Prot entry Q9Y6D9 (GeneID 8379). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
hMAD1, HsMAD1, MAD1, MAD1 mitotic arrest deficient like 1, MAD1-like protein 1, mitotic arrest deficient 1-like protein 1, mitotic checkpoint MAD1 protein homolog, mitotic spindle assembly checkpoint protein MAD1, mitotic-arrest deficient 1, yeast, homolo
between 1 and 50
2 - 8°C
Detection of human MAD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg/lane in A; 50 µg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-MAD1 antibody A300-339A used at 0.1 µg/ml for western blot (A) and at 1 µg/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies BL1689 and BL1690 using 1 µg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using BL1689 at 0.2 µg/ml. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).