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Rabbit anti-MAD1 Antibody, Affinity Purified - A300-339A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MAD1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-339A
Name:
Rabbit anti-MAD1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to MAD1 immobilized on solid support. The epitope recognized by A300-339A maps to a region between residues 1 and 50 of human Mitotic arrest deficient-like 1 using the numbering given in Swiss-Prot entry Q9Y6D9 (GeneID 8379).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
hMAD1, HsMAD1, MAD1, MAD1 mitotic arrest deficient like 1, MAD1-like protein 1, mitotic arrest deficient 1-like protein 1, mitotic checkpoint MAD1 protein homolog, mitotic spindle assembly checkpoint protein MAD1, mitotic-arrest deficient 1, yeast, homolo
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human MAD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody A300-339A (lot A300-339A-1) used for WB at 0.06 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human MAD1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg/lane in A; 50 µg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-MAD1 antibody A300-339A used at 0.1 µg/ml for western blot (A) and at 1 µg/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies BL1689 and BL1690 using 1 µg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using BL1689 at 0.2 µg/ml. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).

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Detection of human MAD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 (Cat. No. A300-339A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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