Antibody was affinity purified using an epitope specific to CTBP1 immobilized on solid support. The epitope recognized by A300-338A maps to a region between residues 400 and 440 of human C-terminal Binding Protein 1 using the numbering given in Swiss-Prot entry Q13363 (GeneID 1487). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
BARS, brefeldin A-ribosylated substrate, CtBP1, C-terminal-binding protein 1, HADDTS
between 400 and 440
2 - 8°C
Detection of human and mouse CTBP1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-CTBP1 antibody A300-338A (lot A300-338A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).