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Rabbit anti-JLP Antibody, Affinity Purified - A300-337A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-JLP Antibody, Affinity Purified
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Size: 100 µg
£302.00
GBP | Euro | USD
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Catalogue number:
A300-337A
Name:
Rabbit anti-JLP Antibody, Affinity Purified
Supplier:
Availability:

Antibody was affinity purified using an epitope specific to JLP immobilized on solid support. The epitope recognized by A300-337A maps to a region between residue 775 and 825 of human JNK/SAPK-Associated Protien using the numbering given in entry NP_003962.3 (GeneID 9043).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
cancer/testis antigen 89, c-Jun NH2-terminal kinase-associated leucine zipper protein, C-Jun-amino-terminal kinase-interacting protein 4, CT89, HLC4, HLC6, HLC-6, human lung cancer oncogene 6 protein, JIP4, JIP-4, JLP, JNK interacting protein, JNK/SAPK-as
Species Reactivity:
Human, Mouse
Immunogen:
Between 775 and 825
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human JLP by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) and HEK293T (50µg) cells. Antibodies: Affinity purified rabbit anti-JLP antibody A300-337A (lot A300-337A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse JLP by western blot. Samples: Whole cell lysate from TCKM (50µg), Renca (50µg), and CT26 (50µg) cells. Antibodies: Affinity purified rabbit anti-JLP antibody A300-337A (lot A300-337A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JLP antibody A300-337A (lot A300-337A-2) used for IP at 6 µg/mg lysate. JLP was also immunoprecipitated by a previous lot (A300-337A-1) of this antibody. For blotting immunoprecipitated JLP, A300-337A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human JLP by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-JLP (Cat. No. A300-337A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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