Antibody was affinity purified using an epitope specific to JLP immobilized on solid support. The epitope recognized by A300-336A maps to a region between residue 1 and 50 of human JNK/SAPK-Associated Protein using the numbering given in entry NP_003962.3 (GeneID 9043). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cancer/testis antigen 89, c-Jun NH2-terminal kinase-associated leucine zipper protein, C-Jun-amino-terminal kinase-interacting protein 4, CT89, HLC4, HLC6, HLC-6, human lung cancer oncogene 6 protein, JIP4, JIP-4, JLP, JNK interacting protein, JNK/SAPK-as
Between 1 and 50
2 - 8°C
Detection of human and mouse JLP by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JLP antibody A300-336A (lot A300-336A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JLP antibody A300-336A (lot A300-336A-3) used for IP at 3 µg per reaction. JLP was also immunoprecipitated by rabbit anti-JLP antibody A300-337A. For blotting immunoprecipitated JLP, A300-336A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.