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Rabbit anti-JLP Antibody, Affinity Purified

Catalogue number:
A300-336A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to JLP immobilized on solid support. The epitope recognized by A300-336A maps to a region between residue 1 and 50 of human JNK/SAPK-Associated Protein using the numbering given in entry NP_003962.3 (GeneID 9043). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

cancer/testis antigen 89, c-Jun NH2-terminal kinase-associated leucine zipper protein, C-Jun-amino-terminal kinase-interacting protein 4, CT89, HLC4, HLC6, HLC-6, human lung cancer oncogene 6 protein, JIP4, JIP-4, JLP, JNK interacting protein, JNK/SAPK-as

Species Reactivity:

Human, Mouse

Immunogen:

Between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse JLP by west

Detection of human and mouse JLP by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JLP antibody A300-336A (lot A300-336A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human JLP by western blot o

Detection of human JLP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JLP antibody A300-336A (lot A300-336A-3) used for IP at 3 µg per reaction. JLP was also immunoprecipitated by rabbit anti-JLP antibody A300-337A. For blotting immunoprecipitated JLP, A300-336A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human JLP by immunohistoche

Detection of human JLP by immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-JLP antibody (A300-336A lot 3) used at 1:1000 (1µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).