Antibody was affinity purified using an epitope specific to TRIP6 immobilized on solid support. The epitope recognized by A300-334A maps to a region between residue 100 and 150 of human Thyroid Hormone Receptor Interactor 6 using the numbering given in entry NP_003293.1 (GeneID 7205). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
OIP1, OIP-1, OPA-interacting protein 1, thyroid hormone receptor interacting protein 6, thyroid receptor-interacting protein 6, TR-interacting protein 6, TRIP-6, TRIP6i2, ZRP-1, zyxin related protein 1, Zyxin-related protein 1
between 100 and 150
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human TRIP6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP6 antibody A300-334A (lot A300-334A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.