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Rabbit anti-FHL2 Antibody, Affinity Purified

Catalogue number:
A300-332A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to FHL2 immobilized on solid support. The epitope recognized by A300-332A maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Detection of human and mouse FHL-2 by we
Product Type:

Antibodies

Alternative Names:

AAG11, aging-associated gene 11, down-regulated in rhabdomyosarcoma LIM protein, DRAL, FHL-2, four and a half LIM domains protein 2, LIM domain protein DRAL, skeletal muscle LIM-protein 3, SLIM3, SLIM-3

Species Reactivity:

Human, Mouse

Immunogen:

between 175 and 225

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse FHL-2 by we

Detection of human and mouse FHL-2 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-FHL-2 antibody A300-332A (lot A300-332A-2) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. FHL-2 was also immunoprecipitated by a previous lot (lot A300-332A-1) of this antibody and by two lots of rabbit anti-FHL-2 antibody A300-333A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).