Antibody was affinity purified using an epitope specific to CoAA immobilized on solid support. The epitope recognized by A300-331A maps to a region between residues 150 and 200 of human Coactivator Activator (RNA Binding Motif Protein 14) using the numbering given in entry NP_006319.1 (GeneID 10432). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
COAA, paraspeckle protein 2, PSP2, RNA-binding motif protein 14, RNA-binding protein 14, RRM-containing coactivator activator/modulator, SIP, synaptotagmin-interacting protein, SYT-interacting protein, SYTIP1, TMEM137, transmembrane protein 137
between 150 and 200
2 - 8°C
Detection of human CoAA by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CoAA antibody A300-331A (lot A300-331A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.