Antibody was affinity purified using an epitope specific to PHD4 immobilized on solid support. The epitope recognized by A300-330A maps to a region between residue 450 and the C-terminus (residue 502) of human Prolyl Hydroxylase Domain-Containing Protein 4 using the numbering given in entry NP_808808.1 (GeneID 54681). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
EGLN4, HIFPH4, HIF-PH4, HIF-prolyl hydroxylase 4, HPH-4, hypoxia-inducible factor prolyl 4-hydroxylase, hypoxia-inducible factor prolyl hydroxylase 4, P4H with transmembrane domain, P4H-TM, PH4, PH-4, PHD4, proline 4-hydroxylase, prolyl 4-hydroxylase, tra
between 450 and C-term
2 - 8°C
Detection of human PHD4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD4 antibody A300-330A (lot A300-330A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human PHD4 by western blot. Samples: Whole cell lysate (25 µg/lane) from human glioblastoma tumor cell lines or in vitro translated PHD4. Antibody: Affinity purified rabbit anti-PHD4 (Cat. No. A300-330A) used at 2 µg/ml. Detection: Chemiluminescence.