Antibody was affinity purified using an epitope specific to PHD3 immobilized on solid support. The epitope recognized by A300-327A maps to a region between residue 50 and 100 of human Prolyl Hydroxylase Domain-Containing Protein 3 using the numbering given in entry NP_071356.1 (GeneID 112399). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
egl nine homolog 3, egl nine-like protein 3 isoform, HIFP4H3, HIFPH3, HIF-PH3, HIF-prolyl hydroxylase 3, HPH-1, HPH-3, hypoxia-inducible factor prolyl hydroxylase 3, PHD3, prolyl hydroxylase domain-containing protein 3
between 50 and 100
2 - 8°C
Detection of human and mouse PHD3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHD3 antibody A300-327A (lot A300-327A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.