Antibody was affinity purified using an epitope specific to RFC4 immobilized on solid support. The epitope recognized by A300-321A maps to a region between residues 1 and 50 of human Replication Factor C subunit 4 using the numbering given in entry NP_002907.1 (GeneID 5984). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1, A1 37 kDa subunit, activator 1 37 kDa subunit, activator 1 subunit 4, replication factor C (activator 1) 4, 37kDa, replication factor C 37 kDa subunit, replication factor C subunit 4, RFC 37 kDa subunit, RF-C 37 kDa subunit, RFC37
between 1 and 50
2 - 8°C
Detection of human RFC4 (aka RFC37) by western blot and immunoprecipitation. Sample: Nuclear extract (20 µg for WB; 300 µg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-RFC4 (Cat. No. A300-321A) used at 0.5 µg/ml for WB and 2 µg/300 µg lysate for IP. Detection: Chemiluminescence with 10 sec. exposure.