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Rabbit anti-Plant Ubiquitin Antibody, Affinity Purified

Catalogue number:
Size:
100 µg _$$_
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Applications:

WB

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

Immunogen:

Between 1 and 50

Product Description:

Antibody was affinity purified using an epitope specific to Ubiquitin immobilized on solid support. The epitope recognized by A300-318A maps to a region between residues 1 and 50 of Arabidopsis thaliana Ubiquitin using the numbering given in Swiss-Prot entry NP_191784.1 (GeneID 825398).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage Temperature:

2 - 8°C

Detection of Plant Ubiquitin by western
Detection of Plant Ubiquitin by western blot. Samples: Recombinant GST-Ubiquitin Antibody: Affinity purified rabbit anti-Plant Ubiquitin antibody (Cat. No. A300-318A) used at 0.4 µg/ml for western blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes.
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