Antibody was affinity purified using an epitope specific to Ubiquitin immobilized on solid support. The epitope recognized by A300-318A maps to a region between residues 1 and 50 of Arabidopsis thaliana Ubiquitin using the numbering given in Swiss-Prot entry NP_191784.1 (GeneID 825398).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Between 1 and 50
2 - 8°C
Detection of Plant Ubiquitin by western blot. Samples: Recombinant GST-Ubiquitin Antibody: Affinity purified rabbit anti-Plant Ubiquitin antibody (Cat. No. A300-318A) used at 0.4 µg/ml for western blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes.