Rabbit anti-PIM1 Antibody, Affinity Purified - A300-313A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to PIM1 immobilized on solid support. The epitope recognized by A300-313A maps to a region between residue 250 and the C-terminus (residue 313) of human PIM1 oncoprotein using the numbering given in Swiss-Prot entry P11309 (GeneID 5292). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human PIM1 by western blot. Samples: Whole cell lysate (50 µg) from K-562 cells. Antibody: Affinity purified rabbit anti-PIM1 antibody (Cat. No. A300-313A) used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 20 seconds.
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