Antibody was affinity purified using an epitope specific to PIM1 immobilized on solid support. The epitope recognized by A300-313A maps to a region between residue 250 and the C-terminus (residue 313) of human PIM1 oncoprotein using the numbering given in Swiss-Prot entry P11309 (GeneID 5292). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Oncogene PIM1, PIM, pim-1 kinase 44 kDa isoform, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1, serine/threonine-protein kinase pim-1
between 250 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide