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Rabbit anti-CDK11 Antibody, Affinity Purified

Catalogue number:
A300-311A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to CDK11 immobilized on solid support. The epitope recognized by A300-311A maps to a region between residues 730 and the C-terminus (residue 780) of human Cell Division Cycle 2-like 2 using the numbering given in SwissProt entry Q9UQ88 (GeneID 728642). The epitope that is recognized by A300-311A is also found in CDC1L1 (Gene ID 984). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

CDC2L2, CDC2L3, CDK11-p110, CDK11-p46, CDK11-p58, cell division cycle 2-like 2 (PITSLRE proteins), cell division cycle 2-like protein kinase 2, cell division protein kinase 11A, cyclin-dependent kinase 11A, galactosyltransferase-associated protein kinase

Species Reactivity:

Human

Immunogen:

between 730 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human CDK11 by western blot

Detection of human CDK11 by western blot. Samples: Whole cell lysate (15 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody A300-311A (lot A300-311A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CDK11 by western blot

Detection of human CDK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK11 antibody A300-311A (lot A300-311A-3) used for IP at 3 µg per reaction. CDK11 was also immunoprecipitated by a previous lot of this antibody (lot A300-311A-2). For blotting immunoprecipitated CDK11, A300-311A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human CDK11 by immunohistoc

Detection of human CDK11 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDK11 (Cat. No. A300-311A Lot 3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB