Antibody was affinity purified using an epitope specific to TAFII68 immobilized on solid support. The epitope recognized by A300-309A maps to a region between residue 550 and the C-terminus (residue 592) of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
68 kDa TATA-binding protein-associated factor, Npl3, RBP56, RBP56/CSMF fusion, RNA-binding protein 56, TAF(II)68, TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa, TAF2N, TAFII68, TATA box binding protein (TBP)-associated f
between 550 and C-term
2 - 8°C
Detection of human TAFII68 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAFII68 antibody A300-309A (lot A300-309A-1) used for WB at 0.033 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human TAFII68 by western blot and immunoprecipitation. Samples: Whole cell lysate (200 µg for WB; 10 mg for IP/WB) from HEK293T cells. Antibodies: Affinity purified rabbit anti-TAFII68 antibody BL1359 (Cat. No. A300-309A) used at 0.05 µg/ml for WB. TAFII68 was immunoprecipitated with rabbit anti-TAFII68 antibodies BL1356 (Cat. No. A300-307A), BL1357 (Cat. No. A300-308A), BL1358 and BL1359 using each at 0.3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 5 seconds.