Antibody was affinity purified using an epitope specific to TAFII68 immobilized on solid support. The epitope recognized by A300-308A maps to a region between residues 200 and 250 of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
68 kDa TATA-binding protein-associated factor, Npl3, RBP56, RBP56/CSMF fusion, RNA-binding protein 56, TAF(II)68, TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa, TAF2N, TAFII68, TATA box binding protein (TBP)-associated f
between 200 and 250
2 - 8°C
Detection of human TAFII68 by western blot and immunoprecipitation. Samples: Whole cell lysate (200 µg for WB; 10 mg for IP/WB) from HEK293T cells. Antibodies: Affinity purified rabbit anti-TAFII68 antibody BL1357 (Cat. No. A300-308A) used at 0.1 µg/ml for WB. TAFII68 was immunoprecipitated with rabbit anti-TAFII68 antibodies BL1356 (Cat. No. A300-307A), BL1357, BL1358 and BL1359 (Cat. No. A300-309A) using each at 0.3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 5 seconds.
Detection of human TAFII68 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 (Cat. No. A300-308A Lot1) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB