Antibody was affinity purified using an epitope specific to KIAA0082 immobilized on solid support. The epitope recognized by A300-305A maps to a region between residue 800 and the C-terminus (residue 835) of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Cap methyltransferase 1, cap1 2'O-ribose methyltransferase 1, cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, FtsJ methyltransferase domain containing 2, ftsJ methyltransferase domain-containing protein 2, FTSJD2, hMTr1, interferon-stimulated ge
between 800 and C-term
2 - 8°C
Detection of human KIAA0082 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIAA0082 antibody A300-305A (lot A300-305A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human KIAA0082 by western blot and immunoprecipitation. Samples: A. Whole cell lysate (T, 15µg; E, 60µg; G, 15µg) from HEK293T cells transfected with a myc-KIAA0082 expression construct (T), HEK293T cells that were mock transfected (E) and G361 cells (G). B. Whole cell lysate (60 µg for Input; 1 mg for IP) from 293T cells. Antibodies: Affinity purified rabbit anti-KIAA0082 antibody BL1522 (Cat. No. A300-305A) used at 0.1 µg/ml for WB (A) and at 1 µg/mg lysate for IP (B). KIAA0082 was also immunoprecipitated using rabbit anti-KIAA0082 antibodies BL1519 (Cat. No. A300-303A) and BL1520 (Cat. No. A300-304A) each used at 1 µg/mg lysate. immunoprecipitatesd KIAA0082 was blotted with BL1520 using 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 5 minutes (A) and 1 minute (B).