Antibody was affinity purified using an epitope specific to KIAA0082 immobilized on solid support. The epitope recognized by A300-304A maps to a region between residues 100 and 150 of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Cap methyltransferase 1, cap1 2'O-ribose methyltransferase 1, cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1, FtsJ methyltransferase domain containing 2, ftsJ methyltransferase domain-containing protein 2, FTSJD2, hMTr1, interferon-stimulated ge
between 100 and 150
2 - 8°C
Detection of human KIAA0082 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIAA0082 antibody A300-304A (lot A300-304A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human and mouse KIAA0082 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse CT26 colon carcinoma (right). Antibody: Affinity purified rabbit anti- KIAA0082 (Cat. No. A300-304A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
Detection of human KIAA0082 by western blot and immunoprecipitation. Samples: Whole cell lysate (60 µg for Input; 1 mg for IP) from HEK293T cells. Antibodies: Affinity purified rabbit anti-KIAA0082 antibody BL1520 (Cat. No. A300-304A) used at 0.1 µg/ml for WB and at 1 µg/mg lysate for IP. KIAA0082 was also immunoprecipitated using rabbit anti-KIAA0082 antibodies BL1519 (Cat. No. A300-303A) and BL1522 (Cat. No. A300-305A) each used at 1 µg/mg lysate. Control antibody is a polyclonal mouse antiserum generated to a recombinant fragment of KIAA0082 used at 1:500 for WB. Detection: Chemiluminescence with exposure times of 5 minutes (Control Ab) and 1 minute (BL1520).