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Rabbit anti-FUS Antibody, Affinity Purified - A300-302A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-FUS Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-302A
Name:
Rabbit anti-FUS Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to FUS immobilized on solid support. The epitope recognized by A300-302A maps to a region between residues 1 and 50 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
75 kDa DNA-pairing protein, ALS6, ETM4, FUS/ERG chimeric protein, FUS/ERG fusion protein, FUS1, fused in sarcoma, fusion gene in myxoid liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, oncogene FUS, oncogene TLS, POMP75,
Species Reactivity:
Human, Mouse
Immunogen:
between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FUS antibody A300-302A (lot A300-302A-4) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human FUS by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FUS antibody A300-302A (lot A300-293A-4) used for IP at 3 µg per reaction. FUS was also immunoprecipitated by rabbit anti-FUS recombinant monoclonal antibody [BLR023E] (A700-023) and rabbit anti-FUS antibody A300-293A. For blotting immunoprecipitated FUS, A700-023 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.

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Detection of human FUS by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS (Cat. No. A300-302A Lot4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of mouse FUS by immunohistochemistry. Sample: FFPE section of mouse renal cells carcinoma. Antibody: Affinity purified rabbit anti-FUS (Cat. No. A300-302A Lot4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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