Antibody was affinity purified using an epitope specific to MAD2 immobilized on solid support. The epitope recognized by A300-300A maps to a region between residues 100 and 150 of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: IP, IHC
HSMAD2, MAD2, MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2 mitotic arrest deficient-like 1, MAD2-like protein 1, mitotic arrest deficient 2-like protein 1, mitotic arrest deficient, yeast, homolog-like 1, mitotic spindle assembly checkpoin
between 100 and 150
2 - 8°C
Detection of human MAD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2 antibody A300-300A (lot A300-300A-3) used for IP at 3 µg/mg lysate. MAD2 was also immunoprecipitated by a previous lot (lot A300-300A-2) and by rabbit anti-MAD2 antibody A300-301A (lot A300-301A-2). Detection: Chemiluminescence with an exposure time of 30 seconds.