Antibody was affinity purified using an epitope specific to MAD2 immobilized on solid support. The epitope recognized by A300-299A maps to a region between residues 2550 and 2600 of human ataxia telangiectasia mutated using the numbering given in SwissProt entry Q13315 (GeneID 472). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
AT mutated, A-T mutated, AT1, ATA, ataxia telangiectasia mutated, ATC, ATD, ATDC, ATE, serine-protein kinase ATM, TEL1, TEL1, telomere maintenance 1, homolog, TELO1
between 2550 and 2600
2 - 8°C
Detection of human ATM by western blot and immunoprecipitation. Samples: A) Whole cell lysate (50 µg) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody: Affinity purified rabbit anti-ATM antibody BL706 (Cat. No. A300-299A) used at the indicated concentrations for WB and at 0.5 µg/mg lysate for IP. immunoprecipitatesd ATM was blotted with a monoclonal antibody to ATM. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human ATM by immunohistochemistry. Sample: FFPE section of human prostate-nodular hypertrophy. Antibody: Affinity purified rabbit anti-ATM (Cat. No. A300-299A) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB