www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-Chk1 Antibody, Affinity Purified

Catalogue number:
A300-298A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£329.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to Chk1 immobilized on solid support. The epitope recognized by A300-298A maps to a region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

cell cycle checkpoint kinase, Checkpoint kinase-1, Checkpoint, S. pombe, homolog of, 1, CHK1, CHK1 checkpoint homolog, Chk1-S, serine/threonine-protein kinase Chk1

Species Reactivity:

Human

Immunogen:

between 250 and 300

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Chk1 by western blot.

Detection of human Chk1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TMCK cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody A300-298A (lot A300-298A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Chk1 by western blot

Detection of human Chk1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Chk1 antibody A300-298A (lot A300-298A-2) used for IP at 6 µg/mg lysate. Chk1 was also immunoprecipitated by a previous lot (lot A300-298A-1) of this antibody. For blotting immunoprecipitated Chk1, A300-298A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CHK1 by immunohistoch

Detection of human CHK1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CHK1 (Cat. No. A300-298A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB