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Rabbit anti-FOXO1a Antibody, Affinity Purified - A300-297A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-FOXO1a Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-297A
Name:
Rabbit anti-FOXO1a Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to FOXO1a immobilized on solid support. The epitope recognized by A300-297A maps to a region between residues 600 and the C-terminus (residue 652) of human Forkhead Box O1a using the numbering given in Swiss-Prot entry Q12778 (GeneID 2308).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
FKH1, FKHR, forkhead box protein O1, forkhead box protein O1A, Forkhead in rhabdomyosarcoma, forkhead, Drosophila, homolog of, in rhabdomyosarcoma, FOXO1A
Species Reactivity:
Human
Immunogen:
Between 600 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human FOXO1a by western blot. Samples: Whole cell lysate from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FOXO1a antibody A300-297A (lot A300-297A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human FOXO1a by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FOXO1a antibody A300-297A (lot A300-297A-3) used for IP at 6 µg per reaction. FOXO1a was also immunoprecipitated by a previous lot of this antibody (lot A300-297A-2). For blotting immunoprecipitated FOXO1a, A300-297A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human FOXO1a by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FOXO1a (Cat. No. A300-297A Lot2) used at a dilution of 1:500 (2µg/ml). Detection: DAB.

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