Antibody was affinity purified using an epitope specific to FUS immobilized on solid support. The epitope recognized by A300-292A maps to a region between residues 200 and 250 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
75 kDa DNA-pairing protein, ALS6, ETM4, FUS/ERG chimeric protein, FUS/ERG fusion protein, FUS1, fused in sarcoma, fusion gene in myxoid liposarcoma, fus-like protein, heterogeneous nuclear ribonucleoprotein P2, HNRNPP2, oncogene FUS, oncogene TLS, POMP75,
between 200 and 250
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse FUS by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FUS antibody A300-292A (lot A300-292A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.