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Rabbit anti-Caper Antibody, Affinity Purified - A300-291A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Caper Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-291A
Name:
Rabbit anti-Caper Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Caper immobilized on solid support. The epitope recognized by A300-291A maps to a region between residues 325 and 375 of human Caper, Coactivator of activating protein-1 and estrogen receptors using the numbering given in SwissProt entry Q14498 (GeneID 9584).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
CAPER, CAPER alpha, CAPERalpha, coactivator of activating protein-1 and estrogen receptors, epididymis secretory sperm binding protein, FSAP59, functional spliceosome-associated protein 59, HCC1, hepatocellular carcinoma protein 1, RNA-binding motif prote
Species Reactivity:
Human
Immunogen:
Between 325 and 375
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Caper by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caper antibody A300-291A (lot A300-291A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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Detection of human Caper by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Caper antibody A300-291A (lot A300-291A-2) used for IP at 6 µg per reaction. Caper was also immunoprecipitated by a previous lot of this antibody (lot A300-291A-1). For blotting immunoprecipitated Caper, A300-291A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Caper by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Caper (Cat. No. A300-291A Lot 2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.

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