Rabbit anti-hSET1 Antibody, Affinity Purified - A300-290A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to hSET1 immobilized on solid support. The epitope recognized by A300-290A maps to a region between residue 1675 and the C-terminus (residue 1709) of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human hSET1 by western blot. Samples: Nuclear extract (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-290A (lot A300-290A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human hSET1 by western blot of immunoprecipitates. Samples: Nuclear extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hSET1 antibody A300-290A (lot A300-290A-2) used for IP at 6 µg per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody A300-290A-1. For blotting immunoprecipitated hSET1, A300-289A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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