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Rabbit anti-hSET1 Antibody, Affinity Purified

Catalogue number:
A300-290A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to hSET1 immobilized on solid support. The epitope recognized by A300-290A maps to a region between residue 1675 and the C-terminus (residue 1709) of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

histone-lysine N-methyltransferase SETD1A, hSET1A, KMT2F, lysine N-methyltransferase 2F, SET domain-containing protein 1A, Set1, set1/Ash2 histone methyltransferase complex subunit SET1, Set1A

Species Reactivity:

Human

Immunogen:

between 1675 and C-term

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human hSET1 by western blot

Detection of human hSET1 by western blot. Samples: Nuclear extract (50 µg) from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-290A (lot A300-290A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human hSET1 by western blot

Detection of human hSET1 by western blot of immunoprecipitates. Samples: Nuclear extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hSET1 antibody A300-290A (lot A300-290A-2) used for IP at 6 µg per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody A300-290A-1. For blotting immunoprecipitated hSET1, A300-289A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.