Antibody was affinity purified using an epitope specific to hSET1 immobilized on solid support. The epitope recognized by A300-290A maps to a region between residue 1675 and the C-terminus (residue 1709) of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
histone-lysine N-methyltransferase SETD1A, hSET1A, KMT2F, lysine N-methyltransferase 2F, SET domain-containing protein 1A, Set1, set1/Ash2 histone methyltransferase complex subunit SET1, Set1A
between 1675 and C-term
2 - 8°C
Detection of human hSET1 by western blot of immunoprecipitates. Samples: Nuclear extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hSET1 antibody A300-290A (lot A300-290A-2) used for IP at 6 µg per reaction. hSET1 was also immunoprecipitated by a previous lot of this antibody A300-290A-1. For blotting immunoprecipitated hSET1, A300-289A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.