Rabbit anti-hSET1 Antibody, Affinity Purified - A300-289A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Detection of human hSET1 by western blot. Samples: Nuclear extract (50, 15, 5 µg) from HEK293T cells. Antibody: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human hSET1 by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-hSET1 antibody A300-289A (lot A300-289A-4) used for IP at 6 µg per reaction. hSET1 was also immunoprecipitated by rabbit anti-hSET1 antibody BL1192. For blotting immunoprecipitated hSET1, A300-289A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human hSet1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-hSet1 Cat. No. A300-289A Lot4 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

ChIP-chip scatter plot of anti-hSET1 (A300-289A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-289A was used to immunoprecipitate chromatin from K-562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitatesd DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-hSET1 ChIP, normal rabbit IgG showed little enrichment.