www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-HIF1-alpha Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (200 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 3 Apr
Shipping is calculated in checkout
Applications:

WB, IP, IHC, ICC-IF

Antibody Host:

Rabbit

Target:

HIF1-alpha

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 775 and C-term

Alternative Names:

HIF1; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); PASD8; PAS domain-containing protein 8; MOP1; member of PAS superfamily 1; member of PAS protein 1; hypoxia-inducible factor1alpha; hypoxia-inducible factor 1-a

image 0
image 1
image 2
Product Description:

Rabbit anti-HIF1-alpha Antibody, Affinity Purified - 100 µl (200 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human HIF1 alpha by western
Detection of human HIF1 alpha by western blot. Samples: Whole cell lysate (15 and 50 µg) from HEK293T cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 3 minutes.
Detection of mouse HIF1 alpha by western
Detection of mouse HIF1 alpha by western blot. Samples: Whole cell lysate (50 µg) from TMCK cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds.
Detection of human HIF1 alpha by western
Detection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells that were treated with 200 µM cobalt chloride. Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 1 µg/ml and for IP at 6 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes.
Detection of human HIF1-alpha by immunoh
Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Affinity purified rabbit anti-HIF1-alpha antibody (Cat. No. A300-286A Lot6) used at a dilution of 1:100 (2µg/ml). Detection: DAB
Detection of human HIF1-alpha by immunoc
Detection of human HIF1-alpha by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells treated with 200 µM cobalt chloride (left) or mock treatment (right). Antibody: Affinity purified rabbit anti-HIF1-alpha antibody Cat. No. A300-286A Lot6 (upper images) and A300-286A Lot5 (lower images) used at a dilution of 1:100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated (A120-201D4). Counterstain: DAPI (blue) and fluorescent phalloidin (green).
Details Cat number & supplier Size Price
SurePAGE™, Bis-Tris, 10x8, 4-12%, 12 wells M00653 · GenScript Biotech
M00653
GenScript Biotech
10 pk/box £107.00
10 pk/box
view
SurePAGE™, Bis-Tris, 10x8, 4-20%, 12 wells M00656 · GenScript Biotech
M00656
GenScript Biotech
10 pk/box £107.00
10 pk/box
view
SurePAGE™, Bis-Tris, 10x8, 10%, 12 wells M00665 · GenScript Biotech
M00665
GenScript Biotech
10 pk/box £107.00
10 pk/box
view