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Rabbit anti-HIF1-alpha Antibody, Affinity Purified - A300-286A - 20 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-HIF1-alpha Antibody, Affinity Purified
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Size: 20 µg
£302.00
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Catalogue number:
A300-286A
Name:
Rabbit anti-HIF1-alpha Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to HIF1-alpha immobilized on solid support. The epitope recognized by A300-286A maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit using the numbering given in Swiss-Prot entry Q16665 (GeneID 3091).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC, ICC-IF

Product Type:
Antibodies
Alternative Names:
ARNT interacting protein, ARNT-interacting protein, basic-helix-loop-helix-PAS protein MOP1, bHLHe78, class E basic helix-loop-helix protein 78, HIF1, HIF-1A, HIF1-ALPHA, HIF-1alpha, HIF-1-alpha, hypoxia inducible factor 1 alpha subunit, hypoxia inducible
Species Reactivity:
Human, Mouse
Immunogen:
Between 775 and C-term
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human HIF1 alpha by western blot. Samples: Whole cell lysate (15 and 50 µg) from HEK293T cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with exposure times of 3 minutes.

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Detection of mouse HIF1 alpha by western blot. Samples: Whole cell lysate (50 µg) from TMCK cells that were either treated with 200 µM cobalt chloride (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with exposure times of 30 seconds.

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Detection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells that were treated with 200 µM cobalt chloride. Antibodies: Affinity purified rabbit anti-HIF1 alpha antibody A300-286A (lot number A300-286A-6) used for WB at 1 µg/ml and for IP at 6 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes.

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Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Affinity purified rabbit anti-HIF1-alpha antibody (Cat. No. A300-286A Lot6) used at a dilution of 1:100 (2µg/ml). Detection: DAB

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Detection of human HIF1-alpha by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells treated with 200 µM cobalt chloride (left) or mock treatment (right). Antibody: Affinity purified rabbit anti-HIF1-alpha antibody Cat. No. A300-286A Lot6 (upper images) and A300-286A Lot5 (lower images) used at a dilution of 1:100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated (A120-201D4). Counterstain: DAPI (blue) and fluorescent phalloidin (green).

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