Antibody was affinity purified using an epitope specific to Rad6 immobilized on solid support. The epitope recognized by A300-281A maps to the N-terminus of human ubiquitin-conjugating enzyme E2A using the numbering given in Swiss-Prot entry P49459 (GeneID 7319). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E2 ubiquitin-conjugating enzyme A, HHR6A, HR6A, MRXS30, MRXSN, RAD6 homolog A, RAD6A, UBC2, ubiquitin carrier protein A, ubiquitin-conjugating enzyme E2 A, ubiquitin-conjugating enzyme E2A, ubiquitin-protein ligase A
2 - 8°C
Detection of human Rad6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody A300-281A (lot A300-281A-1) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human and mouse Rad6 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Rad6 (Cat. No. A300-281A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB