Antibody was affinity purified using an epitope specific to MTA1 immobilized on solid support. The epitope recognized by A300-280A maps to a region between residues 400 and 450 of human Metastasis Associated Protein 1 using the numbering given in Swiss-Prot entry Q13330 (GeneID 9112). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
metastasis associated gene 1 protein, metastasis-associated protein MTA1
between 400 and 450
2 - 8°C
Detection of human and mouse MTA1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTA1 antibody A300-280A (lot A300-280A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human MTA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTA1 antibody A300-280A (lot A300-280A-2) used for IP at 6 µg per reaction. MTA1 was also immunoprecipitated by rabbit anti-MTA1 antibody A300-280A-1. For blotting immunoprecipitated MTA1, A300-280A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.