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Rabbit anti-MTA1 Antibody, Affinity Purified - A300-280A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-MTA1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-280A
Name:
Rabbit anti-MTA1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to MTA1 immobilized on solid support. The epitope recognized by A300-280A maps to a region between residues 400 and 450 of human Metastasis Associated Protein 1 using the numbering given in Swiss-Prot entry Q13330 (GeneID 9112).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
metastasis associated gene 1 protein, metastasis-associated protein MTA1
Species Reactivity:
Human, Mouse
Immunogen:
Between 400 and 450
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse MTA1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTA1 antibody A300-280A (lot A300-280A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human MTA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTA1 antibody A300-280A (lot A300-280A-2) used for IP at 6 µg per reaction. MTA1 was also immunoprecipitated by rabbit anti-MTA1 antibody A300-280A-1. For blotting immunoprecipitated MTA1, A300-280A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human MTA1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- MTA1 (Cat. No. A300-280A Lot2) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB

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