Rabbit anti-KAP-1 Antibody, Affinity Purified

Name: Rabbit anti-KAP-1 Antibody, Affinity Purified
Brand: Bethyl Laboratories, Inc.
SKU: CBSKU_00070572
Price: 436 GBP
Availability: InStock

Catalogue number:

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A300-275A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µl (1000 µg/ml) _$$_

Product is available in:

Currency:
GBP
EUR

£436.00

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Estimated delivery Wed 1 May
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Applications:

WB, IP, IHC

Antibody Host:

Rabbit

Target:

KAP-1

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 650 and 700

Alternative Names:

KRAB [Kruppel-associated box domain]-associated protein 1; RING finger protein 96; Tripartite motif-containing protein 28; transcriptional intermediary factor 1-beta; transcription intermediary factor 1-beta; TIF1-beta; TIF1B; TF1B; RNF96; RING-type E3 ub

Product Description:

Rabbit anti-KAP-1 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody A300-275A (lot A300-275A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody A300-275A (lot A300-275A-4) used for IP at 6 µg per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot A300-275A-3) and rabbit anti-KAP-1 antibody A300-274A. For blotting immunoprecipitated KAP-1, A300-275A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human Kap-1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. A300-275A Lot4 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB

Detection of mouse Kap-1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytomaAntibody: Affinity purified rabbit anti-Kap-1 Cat. No. A300-275A Lot4 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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