Antibody was affinity purified using an epitope specific to KAP-1 immobilized on solid support. The epitope recognized by A300-275A maps to a region between residue 650 and 700 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
E3 SUMO-protein ligase TRIM28, KAP1, KAP-1, KRAB [Kruppel-associated box domain]-associated protein 1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, nuclear corepressor KAP-1, PPP1R157, protein phosphatase 1, regulatory subunit 157, RING
between 650 and 700
2 - 8°C
Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody A300-275A (lot A300-275A-4) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human KAP-1 by western blot of immunoprecipitates. Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KAP-1 antibody A300-275A (lot A300-275A-4) used for IP at 6 µg per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot A300-275A-3) and rabbit anti-KAP-1 antibody A300-274A. For blotting immunoprecipitated KAP-1, A300-275A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human Kap-1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 Cat. No. A300-275A Lot4 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
Detection of mouse Kap-1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytomaAntibody: Affinity purified rabbit anti-Kap-1 Cat. No. A300-275A Lot4 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB