Antibody was affinity purified using an epitope specific to 53BP1 immobilized on solid support. The epitope recognized by A300-272A maps to a region between residues 350 and 400 of human tumor protein p53 binding protein 1 using the numbering given in entry NP_005648.1 (GeneID 7158). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
53BP1, p202, p53-binding protein 1, p53BP1, TDRD30, TP53-binding protein 1, tumor protein 53-binding protein, 1, tumor suppressor p53-binding protein 1
between 350 and 400
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human and mouse 53BP1 by western blot. Samples: Nuclear extract (50 and 5 µg) from HeLa and (15 µg) from mouse TCMK-1 cells. Antibody: Affinity purified rabbit anti-53BP1 antibody A300-272A (lot A300-272A-6) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 antibody A300-272A (lot A300-272A-6) used for IP at 6 µg per reaction. 53BP1 was also immunoprecipitated by rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (A700-011). For blotting immunoprecipitated 53BP1, A700-011 was used at 1:1000. Chemiluminescence with an exposure time of 10 second.