Antibody was affinity purified using an epitope specific INT7 to immobilized on solid support. The epitope recognized by A300-271A maps to a region between residues 850 and 900 of human INT7 using the numbering given in TrEMBL entry Q9NVH2 (GeneID 25896). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C1orf73, INT7, integrator complex subunit 7
between 850 and 900
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human INT7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT7 antibody A300-271A (lot A300-271A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.